Antagonistic dark/light‐induced SigB/SigD, group 2 sigma factors, expression through redox potential and their roles in cyanobacteria
Open Access
- 22 October 2003
- journal article
- Published by Wiley in FEBS Letters
- Vol. 554 (3) , 357-362
- https://doi.org/10.1016/s0014-5793(03)01188-8
Abstract
The expression of group 2 sigma factors is characterized in a cyanobacterium Synechocystis sp. PCC 6803 grown in culture, changing light conditions (white, red and blue light, and darkness), or the presence of drugs (rifampicin, chloramphenicol, DCMU, and DBMIB), and the roles of these sigma factors are elucidated. The expression of dark/light‐induced SigB/SigD was accelerated under opposite redox (oxidation/reduction) states in an electron transport chain of photosynthesis. Expression of the dark‐induced lrtA and light‐induced psbA2/3 transcript was significantly reduced in the sigB and sigD knockout strains, respectively. Abundant amounts of sigB transcript and protein were observed in the sigC knockout strain, implying that SigC represses SigB expression under light. These findings clearly showed that SigB/SigD with another group 2 sigma, SigC, contribute to transcription for a subset of dark/light‐responsive genes in the cyanobacterium. A possible model for SigB/SigD is presented and the potential ability for promoter recognition is also discussed.Keywords
This publication has 44 references indexed in Scilit:
- Light-responsivepsbATranscription Requires the −35 Hexamer in the Promoter and Its Proximal Upstream Element, UPE, in CyanobacteriaBioscience, Biotechnology, and Biochemistry, 2003
- Two Distinct Curved DNAs Upstream of the Light-responsivepsbAGene in a CyanobacteriumBioscience, Biotechnology, and Biochemistry, 2003
- Genome-Wide Dynamic Transcriptional Profiling of the Light-to-Dark Transition inSynechocystissp. Strain PCC 6803Journal of Bacteriology, 2002
- Identification and Inactivation of Three Group 2 Sigma Factor Genes in Anabaena sp. Strain PCC 7120Journal of Bacteriology, 2001
- Functional Elements of the Strong psbAI Promoter of Synechococcus elongatus PCC 7942Journal of Bacteriology, 2001
- Transcriptional and Posttranscriptional Control of mRNA fromlrtA, a Light-Repressed Transcript inSynechococcus sp. PCC 70021Plant Physiology, 1998
- Expression of two alternative sigma factors of Synechococcus sp. strain PCC 7002 is modulated by carbon and nitrogen stressMicrobiology, 1997
- Sequence Analysis of the Genome of the Unicellular Cyanobacterium Synechocystis sp. Strain PCC6803. II. Sequence Determination of the Entire Genome and Assignment of Potential Protein-coding RegionsDNA Research, 1996
- CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choiceNucleic Acids Research, 1994