Fluorescein Provides a Resonance Gate for FRET from Conjugated Polymers to DNA Intercalated Dyes
- 3 April 2004
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 126 (17) , 5446-5451
- https://doi.org/10.1021/ja035550m
Abstract
Fluorescence spectra show that excitation of the cationic water-soluble conjugated polymer poly{(1,4-phenylene)-2,7-[9,9-bis(6‘-N,N,N-trimethylammonium)-hexyl]fluorene diiodide} (1) results in inefficient fluorescence resonance energy transfer (FRET) to ethidium bromide (EB) intercalated within double-stranded DNA (dsDNA). When fluorescein (Fl) is attached to one terminus of the dsDNA, there is efficient FRET from 1 through Fl to EB. The cascading energy-transfer process was examined mechanistically via fluorescence decay kinetics and fluorescence anisotropy measurements. These experiments show that the proximity and conformational freedom of Fl provide a FRET gate to dyes intercalated within DNA which are optically amplified by the properties of the conjugated polymer. The overall process provides a substantial improvement over previous homogeneous conjugated polymer based DNA sensors, namely, in the form of improved selectivity.Keywords
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