Studies on the Intracellular Amino Acids of Penicillium roqueforti

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Abstract
The intracellular amino-acid pool of the mycelium of Penicillium roqueforti, Minnesota strain P 108-2, comprises 5 to 15% of the total cell nitrogen. Analyses by paper chromatography showed that alanine is present in the highest concentration, with glutamic and aspartic acids in slightly lower amounts. Other amino acids commonly found in protein were present in still smaller concentrations. Nitrogen starvation resulted in increased pool levels, whereas incubation in the presence of a solution of several purine and pyrimidine bases, i.e., adenine, guanine, xanthine, thymine, cytosine and uracil, resulted in decreased pool levels. The respiration inhibitors sodium azide, sodium arsenate and 2,4-dinitrophenol caused the pool levels to decrease.