Photoelectric Method for Estimating the Concentration of Sperm in Boar Semen1

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Abstract
The turbidometric method for estimating sperm concentration in ram and bull semen was adapted to boar semen using a Cenco-Sheard-Sanford Photelometer. Correlations of 0.95 or higher were obtained between hemocytometer counts of sperm concentration and Photelometer readings expressed as 2 — log of the Photelometer reading. Semen dilution rates of 1:9 and 1:19 gave readings, most of which were within the more sensitive range on the Photelometer scale. No significant differences were found between the average sperm counts made by the hemocytometer, or between transmittancy values of samples taken from the top and the bottom of the ejaculate on day 0 and day 1. The regression equations for estimating sperm concentration (Ŷ) in millions per ml. in boar semen were, for the 1:9 dilution rate, Ŷ=862.1X−72.4, and for the 1:19 dilution rate, Ŷ=1328.9X−21.0, where X equals 2 — log of the Photelometer reading. As with ram and bull semen this photelometric procedure saves considerable time in evaluating boar semen. Copyright © . .