Demonstration of multiple forms of bovine brain myristoyl CoA: protein N-myristoyl transferase
- 1 July 1992
- journal article
- Published by Springer Nature in Molecular and Cellular Biochemistry
- Vol. 113 (1) , 77-81
- https://doi.org/10.1007/bf00230888
Abstract
Four distinct N-myristoyl transferase (NMT) activity peaks, designated I, II, III, and IV, were separated from the cytosolic fraction of bovine brain by DEAE-Sepharose column chromatography. Peaks I, II, III and IV were characterised biochemically with respect to substrate specificity; with cAMP-dependent protein kinase and pp60src derived peptides, and by their apparent molecular mass. The apparent molecular mass of peaks I, II, III and IV were 190 kDa, 224 kDa, 390 kDa and 76 kDa, respectively. These results indicate that bovine brain contains multiple forms of NMT.Keywords
This publication has 18 references indexed in Scilit:
- N-myristoyl transferase assay using phosphocellulose paper bindingAnalytical Biochemistry, 1991
- Characterisation of a Myristoyl CoA:Glycylpeptide N‐Myristoyl Transferase Activity in Rat Brain: Subcellular and Regional DistributionJournal of Neurochemistry, 1990
- Detection of myristoyl CoA:protein N-myristoyltransferase activity by ion-exchange chromatographyAnalytical Biochemistry, 1989
- THE BIOLOGY AND ENZYMOLOGY OF EUKARYOTIC PROTEIN ACYLATIONAnnual Review of Biochemistry, 1988
- Myristoylated alpha subunits of guanine nucleotide-binding regulatory proteins.Proceedings of the National Academy of Sciences, 1987
- Hydroxylamine-stable covalent linkage of myristic acid in Goα, a guanine nucleotide-binding protein of bovine brainBiochemical and Biophysical Research Communications, 1987
- Purification and characterization of yeast myristoyl CoA:protein N-myristoyltransferase.Proceedings of the National Academy of Sciences, 1987
- Myristylation site in Pr65gag is essential for virus particle formation by Moloney murine leukemia virus.Proceedings of the National Academy of Sciences, 1986
- Identification of the NH2‐terminal blocking group of calcineurin B as myristic acidFEBS Letters, 1982
- n-Tetradecanoyl is the NH2-terminal blocking group of the catalytic subunit of cyclic AMP-dependent protein kinase from bovine cardiac muscle.Proceedings of the National Academy of Sciences, 1982