An abundant ubiquitous glycoprotein (GP100) in nucleated mammalian cells
Open Access
- 7 January 1985
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 179 (2) , 294-298
- https://doi.org/10.1016/0014-5793(85)80537-8
Abstract
Two‐dimensional gel electrophoresis with the 125I‐Con A overlay and affinity purification with Con A‐agarose revealed the presence of an abundant ubiquitous 100‐kDa glycoprotein (GP100) in nucleated mammalin cells. The amount in cultured human and murine cells varies from 3 to 20 × 106 molecules per cell making GP100 the most abundant glycoprotein in nucleated cells. Peptide mapping shows that it is different from erythrocyte Band III protein. Several properties of GP100 suggest that it could play a structural role in nucleated cell membranes.Keywords
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