MITOCHONDRIA FROM LIPOMYCES LIPOFER

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Abstract
Protoplasts of Lipomyces lipofer were ruptured by decompression in a French pressure cell. A particulate fraction sedimenting at 17,000 × g in saline or sucrose media contained the bulk of the substrate-dependent oxidative activity and was capable of phosphorylation. Particle fractions isolated in sucrose required supplementation with ATP and Mg2+while fractions from saline isolations required, in addition, NAD and cytochrome c. NADP and thiamine pyrophosphate had small or negligible effects in the presence of the other cofactors. Oxidative phosphorylation occurred most efficiently in particles isolated in sucrose, but in no case did the P/O ratio exceed 1.6. Electron microscopic examination of the sucrose-isolated fraction showed it to be a relatively homogeneous preparation of mitochondria which appear more "native" after incubation with substrate than immediately after isolation.

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