Evaluation of Enzyme-Linked Immunosorbent Assay (ELISA) for Mumps Virus Antibodies

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Abstract
Pre- and post-vaccination sera from [human] individuals who were vaccinated with live Jeryl-Lynn vaccine were assayed for mumps antibodies [Ab] by using serum neutralization (NT), enzymed-linked immunosorbent assay (ELISA), hemolysis-in gel (HIG), hemagglutination inhibition (HI) and complement fixation (CF) techniques. ELISA was equally specific and somewhat more sensitive than NT. HIG was less sensitive than either of these two; with some specimens it was less specific. It was more sensitive than HI. CF gave several low positive readings interpreted as false positive, since they were found in pre-vaccination sera, and other tests failed to detect Ab in these samples. The ELISA mumps test can be used in place of the more expensive and time-comsuming neutralization test for screening epidemiology, documenting immunity and establishing serological diagnosis.