Observation of the FeIV=O stretching Raman band for a thiolate‐ligated heme protein Compound I of chloroperoxidase
- 6 July 1992
- journal article
- Published by Wiley in FEBS Letters
- Vol. 305 (3) , 206-208
- https://doi.org/10.1016/0014-5793(92)80668-7
Abstract
The FeIV=O stretching vibration has never been identified for a cysteine-coordinated heme enzyme. In this study, resonance Raman and visible absorption spectra were observed simultaneously for transient species in the catalytic reaction of chloroperoxidase with hydrogen peroxide by using our original apparatus for mixed-flow and Raman/absorption simultaneous measurements. For the first intermediate, the FeIV=O stretching Raman band was observed at 790 cm−1, which shifted to 756 cm−1 with the 18O derivative, but the ν4 band was too weak to be identified. This suggested the formation of an oxoferryl porphyrin π cation radical. The second intermediate gave an intense ν4 band at 1,372 cm−1 but no oxygen isotope-sensitive Raman band, suggesting oxygen exchange with bulk water.Keywords
This publication has 30 references indexed in Scilit:
- Time-Resolved Resonance Raman Investigation of Cytochrome Oxidase Catalysis: Observation of a New Oxygen-Isotope Sensitive Raman BandBulletin of the Chemical Society of Japan, 1991
- Resonance Raman characterization of ferric and ferryl porphyrin .pi. cation radicals and the FeIV:0 stretching frequencyJournal of the American Chemical Society, 1991
- Resonance Raman pursuit of the change from iron(II)-oxygen (FeII-O2) to iron(III)-hydrohxyl (FeIII-OH) via iron(IV):oxygen (FeIV:O) in the autoxidation of ferrous iron-porphyrinJournal of the American Chemical Society, 1990
- Novel optical device for simultaneous measurements of Raman and absorption spectra: Application to photolabile reaction intermediates of hemeproteinsReview of Scientific Instruments, 1988
- Oxygen exchange between the Fe(IV)O heme and bulk water for the A2 isozyme of horseradish peroxidaseFEBS Letters, 1986
- Resonance Raman studies of isotopically labeled chloroperoxidaseBiochemistry, 1986
- Spectra of chloroperoxidase Compounds II and IIIBiochemical and Biophysical Research Communications, 1985
- Observation of the FeIVO stretching vibration of ferryl myoglobin by resonance Raman spectroscopyBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1985
- Resonance Raman spectroscopic characterizations of horseradish peroxidase. Observations of the FeIV = O stretching vibration of Compound IIBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1985
- Spectrum of chloroperoxidase compound IBiochemical and Biophysical Research Communications, 1980