Purification and characterization of DNA methyltransferases fromNeisseria gonorrhoeae
Open Access
- 11 July 1988
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 16 (13) , 5957-5972
- https://doi.org/10.1093/nar/16.13.5957
Abstract
Three DNA methyltransferases, M NgoAI, and M NgoBI and M NgoBII, free of any nuclease activities were isolated from Neisseria gonorrhoeae strains WR220 and MUG116 respectively. M NgoAI recognizes the sequence 5′ GGCC 3′ and methylates the first 5′ cytosine on both strands. M NgoBI and M.NgoBII recognize 5′ TCACC 3′ and 5′ GTAN5CTC 3′ and 5′ GTAN5CTC 3′ respectively. M NgoBII methylates cytosine on only one strand to produce 5′ GTAN5mCTC 3′.This publication has 29 references indexed in Scilit:
- DNA METHYLATION IN ESCHERICHIA COLIAnnual Review of Genetics, 1987
- Phase variation in Bordetella pertussis is accompanied by changes in DNA modificationMicrobial Pathogenesis, 1987
- MISMATCH REPAIR IN ESCHERICHIA COLIAnnual Review of Genetics, 1986
- Alteration of apparent restriction endonuclease recognition specificities by DNA methylasesNucleic Acids Research, 1984
- Characterization of a chimeric β-lactamase plasmid of Neisseria gonorrhoeae which can function in Escherichia coliMolecular Genetics and Genomics, 1983
- Nucleotide sequence of bacteriophage λ DNAJournal of Molecular Biology, 1982
- HineI is an isoschizomer of HinfIII restriction endonucleaseJournal of Molecular Biology, 1982
- The DNA sequence recognised by the HinfIII restriction endonucleaseJournal of Molecular Biology, 1981
- DNA recognition and cleavage by the EcoP15 restriction endonucleaseJournal of Molecular Biology, 1979
- DNA methylases of Hemophilus influenzae Rd: I. Purification and propertiesJournal of Molecular Biology, 1973