A Multi‐channel System for the Measurement of Spermatozoan Collective Motility

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Abstract
A new multi-channel optical system for objective evaluation of sperm motility is described. The principle of the technique is based on the measurement of changes in the intensity of light scattered from semen samples thermoregulated at 38.degree. C. These changes were recorded continuously as analog waves (reflectospermiogram). Changes in the intensity of sperm motility as a function of time from ejaculation were simultaneously measured in 8 equal semen samples [ram] from a single ejaculate. By analyzing the variations in results, the sensitivity of the technique was defined. To demonstrate the use of the technique, the effect of various ethanol concentrations on sperm motility was described. The exposure of sperm cells to 366 nm light used in the system did not alter their ultrastructure, as was observed by EM. This objective, relatively easy to perform and highly informative technique can be of great value in research studies.

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