A synthetic approach to structure-function relationships in the murine epidermal growth factor molecule.
- 1 September 1986
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 83 (17) , 6367-6371
- https://doi.org/10.1073/pnas.83.17.6367
Abstract
Murine epidermal growth factor, a 53-amino acid peptide that is mitogenic for a number of cell types, has been synthesized by the solid-phase method. The synthetic peptide is identical to the natural material in amino acid composition, chromatographic behavior, receptor binding, and stimulation of DNA synthesis. Fragments of the EGF molecule corresponding to residues 42-53, 32-53, and 15-53 were constructed as well as the methionine sulfoxide derivative of EGF, [Met(O)21] EGF-(1-53), and a polymeric form of EGF. [Met(O)21]EGF-(1-53) was slightly less active than EGF in receptor binding and stimulation of DNA synthesis. Polymeric EGF was 1/100th as active as EGF, while EGF-(15-53) was less potent than EGF-(1-53) by a factor of 104. EGF-(32-53) was even less active and EGF-(43-53) was inactive.This publication has 22 references indexed in Scilit:
- Epidermal growth factor from the mouse. Physical evidence for a tiered .beta.-sheet domain: two-dimensional NMR correlated spectroscopy and nuclear overhauser experiments on backbone amide protonsBiochemistry, 1985
- A synthetic fragment of rat transforming growth factor α with receptor binding and antigenic propertiesBiochemical and Biophysical Research Communications, 1985
- Studies on peptides. CXXVII. Synthesis of a tripentacontapeptide with epidermal growth factor activity.CHEMICAL & PHARMACEUTICAL BULLETIN, 1985
- Epidermal growth factor from the mouse. Structural characterization by proton nuclear magnetic resonance and nuclear Overhauser experiments at 500 MHzBiochemistry, 1984
- Biologically active synthetic fragments of epidermal growth factor: localization of a major receptor-binding region.Proceedings of the National Academy of Sciences, 1984
- Structure of a Mouse Submaxillary Messenger RNA Encoding Epidermal Growth Factor and Seven Related ProteinsScience, 1983
- Nucleotide sequence of epidermal growth factor cDNA predicts a 128,000-molecular weight protein precursorNature, 1983
- Quantitative monitoring of solid-phase peptide synthesis by the ninhydrin reactionAnalytical Biochemistry, 1981
- Epidermal Growth Factor1979
- Empirical Predictions of Protein ConformationAnnual Review of Biochemistry, 1978