Autoradiographic Demonstration of DNA Synthesis in Selected Epithelial Tissues in Male Rats

Abstract
Eleven white male rats received intravenous injections of thymidine -3H at a dose rate of 1 μc/gm body weight. The rats were killed at four-hour intervals over a 24-hour period Epithelial specimens from the hard and soft palate, cheek, skin, and tail were subjected to autoradiography. The results confirm prior data from liquid scintillation studies, which showed a maximal DNA synthesis at 4:30 to 8:45 AM and minimal synthesis at 3:40 to 8:30 PM.