Improved Method for the Isolation and Properties of Tuberculin Active Peptides

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Abstract
The cells of tubercle bacilli (human type, Aoyama B strain) were defatted with acetone alcohol and ether, the peptides extracted with 0.1 N HCl at 37[degree]C, dialyzed, precipitated by adding picric acid, dissolved by the addition of 1 N NaOH and by mixing with an equal volume of acetone. The isolated peptide contained 14.7% N, but it was free of any sugar components, and was homogeneous in sedimentation pattern. The peptide preparation gave at least 4 active fractions as demonstrated by DEAE-cellulose and CM-cellulose column chromatographies. Tuberculin potency of Old Tuberculin at 200-fold dilution corresponded to 0.25 gamma of the isolated tuberculin peptide.