An LRR Receptor Kinase Involved in Perception of a Peptide Plant Hormone, Phytosulfokine
- 24 May 2002
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 296 (5572) , 1470-1472
- https://doi.org/10.1126/science.1069607
Abstract
The sulfated peptide phytosulfokine (PSK) is an intercellular signal that plays a key role in cellular dedifferentiation and proliferation in plants. Using ligand-based affinity chromatography, we purified a 120-kilodalton membrane protein, specifically interacting with PSK, from carrot microsomal fractions. The corresponding complementary DNA encodes a 1021–amino acid receptor kinase that contains extracellular leucine-rich repeats, a single transmembrane domain, and a cytoplasmic kinase domain. Overexpression of this receptor kinase in carrot cells caused enhanced callus growth in response to PSK and a substantial increase in the number of tritium-labeled PSK binding sites, suggesting that PSK and this receptor kinase act as a ligand-receptor pair.Keywords
This publication has 16 references indexed in Scilit:
- Diversity of Arabidopsis Genes Encoding Precursors for Phytosulfokine, a Peptide Growth FactorPlant Physiology, 2001
- 120- and 160-kDa Receptors for Endogenous Mitogenic Peptide, Phytosulfokine-α, in Rice Plasma MembranesJournal of Biological Chemistry, 2000
- The Endogenous Sulfated Pentapeptide Phytosulfokine-α Stimulates Tracheary Element Differentiation of Isolated Mesophyll Cells of Zinnia1Plant Physiology, 1999
- Characterization of specific binding sites for a mitogenic sulfated peptide, phytosulfokine‐α, in the plasma‐membrane fraction derived from Oryza sativa L.European Journal of Biochemistry, 1999
- Preparation and Characterization of Fully Active Biotinylated Analogs of Phytosulfokine-αBioscience, Biotechnology, and Biochemistry, 1999
- A Putative Leucine-Rich Repeat Receptor Kinase Involved in Brassinosteroid Signal TransductionCell, 1997
- Active Fragments and Analogs of the Plant Growth Factor, Phytosulfokine: Structure–Activity RelationshipsBiochemical and Biophysical Research Communications, 1996
- The leucine-rich repeat: a versatile binding motifTrends in Biochemical Sciences, 1994
- Characterization of Conditioning Factors that Increase Colony Formation from «Black Mexican Sweet Corn» ProtoplastsJournal of Plant Physiology, 1988
- Conditioning factor affecting growth in plant cells in culturePlant Science, 1987