The electrophoresis of histones in polyacrylamide gel and their quantitative determination
- 1 July 1967
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 104 (1) , 78-82
- https://doi.org/10.1042/bj1040078
Abstract
1. A new method has been devised for the separation of the histone fractions of calf thymus by electrophoresis in polyacrylamide gel at pH2.4. 2. The fractions have been characterized by their relative mobilities with respect to a marker protein, bovine plasma albumin. 3. A method has been developed for the quantitative determination of the separated histone fractions by measuring the colour yields of dye-histone complexes formed in the gel.This publication has 16 references indexed in Scilit:
- Countercurrent-distribution studies on histonesBiochemical Journal, 1967
- Studies on nuclear proteins II. Quantitative distribution of histone fractions in various tissuesBiochimica et Biophysica Acta (BBA) - General Subjects, 1966
- High-resolution disc electrophoresis of histonesAnalytical Biochemistry, 1966
- Some aspects of the use of “continuous” and “discontinuous” buffer systems in polyacrylamide gel electrophoresisAnalytical Biochemistry, 1965
- Studies on histones. 7. Preparative methods for histone fractions from calf thymusBiochemical Journal, 1964
- The presence of acetyl groups in histonesBiochemical Journal, 1963
- Disk Electrophoresis of Basic Proteins and Peptides on Polyacrylamide GelsNature, 1962
- Observations on the species and tissue specificity of histonesBiochemical Journal, 1962
- On the Complexity of Calf Thymus Histone*Biochemistry, 1962
- The electrophoresis of histones and histone fractions on starch gelBiochemical Journal, 1961