A STREPTOMYCIN DEPENDENT LIVE PASTEURELLA-MULTOCIDA VACCINE FOR THE PREVENTION OF RABBIT PASTEURELLOSIS

  • 1 January 1980
    • journal article
    • research article
    • Vol. 30  (3) , 515-518
Abstract
A culture of P. multocida was isolated from a rabbit exhibiting clinical signs of respiratory pasteurellosis. Organisms from this culture were incubated in the presence of the mutagen N-methyl-N''-nitro-N-nitrosoguanidine to obtain a streptomycin dependent mutant. A liver vaccine was prepared from this mutant and healthy Pasteurella-free rabbits were vaccinated intranasally or s.c. Either method provided complete protection against homologous, wild-type challenge as evidence by absence of clinical signs or gross pathology and failure to isolate the organism from mucous secretions or tissues from vaccinated individuals.