Sensitization, Immunological Tolerance and Desensitization of Guinea Pigs to Neoarsphenamine II.

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Abstract
The induction of an "early" or "protracted" type of sensitlzation to neoarsphenamine (NEO) in guinea pigs, as previously described, depends at least on 2 factors, namely the immunogenicity of the NEO lot used and/or the quality of the diet fed. With strong immunogenic lots early sensitization is achieved independently of the quality of the diet, the influence of nutritional factors on sensitization becoming only manifest when weak immunogenic lots are used. As described previously, a diet poor in vitamin C favours early sensitization, a fact, verified by analysis of the fodder and by vitamin C elimination studies in the animals. With weak immunogenic NEO lots and non deficient diet the addition of Freund''s complete adjuvant, the administration of booster injections or the use of altered NEO solutions oxidized by standing, had no effect on the protracted course of sensitlzation usually observed. For strong as well as for weak immunogenic NEO lots one intradermal injection in the range of 150 to 500 ng was found optimal for sensitization, lower doses inducing protracted sensitization, and higher doses immunological tolerance.