Dna synthesis in vitro during contact sensitivity in the mouse

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Abstract
A system is presented for the determination of DNA synthesis in vitro during contact sensitivity to picryl chloride in the mouse.Lymph node cells from mice immunized by skin painting with picryl chloride responded in culture to various 2,4,6‐trinitrophenyl (picryl) protein conjugates with increased incorporation of [14C]thymidine.Some detail was devoted to defining the optimal conditions for culture and it is suggested that this technique would be useful to investigate whether responding cells in culture are thymus (T) or bone marrow (B)‐derived, the importance of hapten: carrier substitution ratios for cell triggering, and the effects of induction of immunological unresponsiveness.