Cre reporter mouse expressing a nuclear localized fusion of GFP and β‐galactosidase reveals new derivatives of Pax3‐expressing precursors
- 7 April 2008
- Vol. 46 (4) , 200-204
- https://doi.org/10.1002/dvg.20384
Abstract
A new Cre‐reporter strain of mouse has been developed that expresses a fusion protein derived from the lacZ gene fused to GFP with a nuclear localization signal. This construct is expressed from the ROSA26 locus upon Cre‐mediated recombination that removes a loxP‐flanked PGK‐neo cassette, thus allowing for detection of Cre activity in all tissues. This reporter strain, which is similar to prior R26R and R26EGFP strains, has certain advantages related to the nuclear expression and the combined expression of both β‐galactosidase and GFP activities. We show that the use of this newly developed reporter line allows for enhanced resolution, detection and co‐localization. Thus, we report a previously unrecognized subset of venous endothelial cells derived from Pax3 expressing precursors. genesis 46:200–204, 2008.Keywords
This publication has 22 references indexed in Scilit:
- Abnormal venous and arterial patterning in chordin mutantsDevelopmental Dynamics, 2007
- Faithful activation of an extra‐bright red fluorescent protein in “knock‐in” Cre‐reporter mice ideally suited for lineage tracing studiesEuropean Journal of Immunology, 2006
- Smooth muscle of the dorsal aorta shares a common clonal origin with skeletal muscle of the myotomeDevelopment, 2006
- Optimized β-galactosidase staining method for simultaneous detection of endogenous gene expression in early mouse embryosGenesis, 2006
- Insertion of Cre into the Pax3 locus creates a new allele of Splotch and identifies unexpected Pax3 derivativesDevelopmental Biology, 2005
- The neural crest is contiguous with the cardiac conduction system in the mouse embryo: a role in induction?Brain Structure and Function, 2004
- BMP receptor IA is required in mammalian neural crest cells for development of the cardiac outflow tract and ventricular myocardiumDevelopment, 2004
- Identification of minimal enhancer elements sufficient for Pax3 expression in neural crest and implication of Tead2 as a regulator of Pax3Development, 2004
- Pax3 is required for enteric ganglia formation and functions with Sox10 to modulate expression of c-retJournal of Clinical Investigation, 2000
- A Novel Transgenic Technique That Allows Specific Marking of the Neural Crest Cell Lineage in MiceDevelopmental Biology, 1999