Expression of c-fos protooncogene in normal human peripheral blood granulocytes.
Open Access
- 1 April 1987
- journal article
- research article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 165 (4) , 1224-1229
- https://doi.org/10.1084/jem.165.4.1224
Abstract
We have investigated by Northern blot analysis the expression of c-fos protooncogene in human peripheral blood polymorphonuclear leukocytes (PMN). Freshly isolated PMN, unlike highly purified circulating lymphoid cells, showed high levels of c-fos transcripts. Appreciable c-fos mRNA was detected in monocytes, but in lesser amounts compared with PMN. Upon exposure to a series of agents that functionally stimulate granulocytes (PMA, inactivated streptococci, TNF, granulocyte and granulocyte/macrophage colony-stimulating factor), a considerable increase in c-fos transcripts was detected. Expression of c-fos in PMN was superinduced by exposure to cycloheximide. These data indicate that the myelomonocytic differentiation pathway c-fos expression is not peculiar to monocytes/macrophages and that PMN may represent a suitable system with which to investigate the link between protooncogene expression and functional activation.This publication has 13 references indexed in Scilit:
- Expression of cellular oncogenes in primary cells from human acute leukemias.Proceedings of the National Academy of Sciences, 1986
- c-fos expression is neither sufficient nor obligatory for differentiation of monomyelocytes to macrophagesCell, 1986
- Activation of human polymorphonuclear neutrophil functions by interferon-gamma and tumor necrosis factors.The Journal of Immunology, 1985
- Human granulocyte-macrophage colony-stimulating factor is a neutrophil activatorNature, 1985
- Rapid induction of the expression of proto-oncogene fos during human monocytic differentiationCell, 1985
- Stimulation of 3T3 cells induces transcription of the c-fos proto-oncogeneNature, 1984
- Differentiation of F9 teratocarcinoma stem cells after transfer of c-fos proto-oncogenesNature, 1984
- Expression of myb, myc and fos proto-oncogenes during the differentiation of a murine myeloid leukaemiaNature, 1984
- Rapid killing of actinomycin D-treated tumor cells by human mononuclear cells. I. Effectors belong to the monocyte-macrophage lineage.The Journal of Immunology, 1984
- RNA Metabolism in Normal and Leukaemic Leucocytes: Further Studies on RNA SynthesisBritish Journal of Haematology, 1968