Studies on glucosaminidase. N-Acetyl-β-glucosaminidase in rat kidney
- 1 March 1957
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 65 (3) , 464-469
- https://doi.org/10.1042/bj0650464
Abstract
The hydrolysis of phenyl N-acetyl-[beta]-D-glucosaminide by N-acetyl-[beta]-glucosaminidase in a partially purified extract of rat kidney was investigated, and conditions for the estimation of the enzyme were established. In 0.05 [image] citrate buffer, the enzyme shows optimum activity at pH 4.3. The optimum substrate concentration cannot be attained because of the limited solubility of the substrate. N-Acetyl-[beta]-glucosaminidase is inhibited competitively by N-acetylglucosamine, acetamide and acetate.Keywords
This publication has 6 references indexed in Scilit:
- ENZYMATIC FORMATION OF MONOSACCHARIDES FROM HYALURONATEJournal of Biological Chemistry, 1955
- Studies in immunochemistry. 11. The action of dilute alkali on the N-acetylhexosamines and the specific blood-group mucoidsBiochemical Journal, 1952
- ALPHA-GLUCOSAMINIDASE1951
- The β-glucosaminase activity of testicular extracts and its bearing on the problem of diffusing factorsBiochemical Journal, 1941
- The hydrolysis of glucosaminides by an enzyme in Helix pomatiaBiochemical Journal, 1939
- The Determination of Enzyme Dissociation ConstantsJournal of the American Chemical Society, 1934