EFFECT OF INTERFERON ON COLONY FORMATION IN CULTURE BY BLAST CELL PROGENITORS IN ACUTE MYELOBLASTIC-LEUKEMIA

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  • 1 January 1980
    • journal article
    • research article
    • Vol. 56  (3) , 549-552
Abstract
The effect of purified human fibroblast interferon on primary and secondary colony formation by blast progenitors from the peripheral blood of patients with acute myelogenous leukemia was examined. Interferon inhibited blast progenitors and normal granulocyte/macrophage progenitors (CFU-C) in a dose-dependent manner. The magnitude of this effect on blast progenitors and CFU [colony forming units] was similar. Interferon also inhibited secondary plating of blast progenitors (self-renewal). This effect was in marked contrast to the effect of adriamycin, which reduced primary plating efficiency of blast progenitors, but did not affect self-renewal. Inhibition of blast progenitor, proliferation by interferon was markedly reduced when interferon was added after 24 h of culture and, was absent when added after 72 h. Inhibition of self-renewal was observed even when interferon was added at 72 h. Interferon inhibits primary proliferation and self-renewal of blast progenitors, and this effect is not due to reduction in the number of primary colonies. These experiments provide an example of how cell culture techniques may be used to test antitumor agents for effects on important cellular events other than general cytotoxicity.