Factor VIII concentrate prepared from blood donors stimulated by intranasal administration of a vasopressin analogue

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Abstract
With an interval of 8 wk between collections, blood was drawn twice from 120 blood donors. At 1 of the donations, 0.25 ml of synthetic vasopressin (DDAVP, 1300 .mu.g/ml), was administered intranasally 60 min prior to collection of the blood. No drug was given at the other donation. The yield of factor VIII clotting activity (VIII:C) and factor VIII antigen (VIIIR:Ag) was compared in blood drawn from the treated and untreated donors. To prevent degradation of VIII:C by fibrinolysis, tranexamic acid was added to the plasma from treated donors immediately after separation from the red blood cells. Plasma from treated donors and the derived Cohn fraction I-O contained approximately twice as much VIII:C as plasma and fraction I-O from untreated control donors. The concentration of VIIIR:Ag was also increased in fraction I-O made from plasma from treated donors, however, to a lesser degree. The in vivo properties of factor VIII concentrates made from each group of donors were studied. Half-life in plasma and recovery of VIII:C were identical. Thus, intranasal synthetic vasopressin may be used to increase the yield of VIII:C in production of factor VIII concentrates.