Effects of Erythropoietin on 14C-Formate Uptake by Spleen and Bone Marrow Nucleic Acids of Erythrocyte-Transfused Mice.

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Abstract
Erythropoietin [EP] increased the specific activity of nuclear and cytoplasmic RNA between 1 and 2 hr. after injection of the hormone. Specific activity of nuclear RNA remains high for 74 hr. while that of cytoplasmic RNA drops between 50 and 74 hr. after EP administration. Specific activity of DNA begins to increase slowly at 8 hr. and then more rapidly at 26 hr. after EP to reach a maximum at 50 hr. Experiments with inactive EP suggest that the effect on nucleic acid metabolism is due to the hormone and not to other substances contained in the erythropoietic preparations. In a condition such as thot of erythroid tissue of the transfused mouse, where only the differentiating effect of EP is being studied, the earliest effect observed is an increase of the uptake of 14C-formate into nRNA, followed later by increased uptake into DNA, which per-sumably reflects the increase of erythroid cells in S phase during the erythropoietic wave that follows EP injection. Further studies are required to determine the nature and significance of the RNA synthesized, soon after EP injection.