Increase of malignant potential of BHK-21 cells by SV40 DNA without persistent new antigen.

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Abstract
BHK-21/C13 hamster fibroblasts exposed to SV40 DNA(deoxy-ribonucleic acid) plated with greater efficiency in agar and on glass than control cells. Cell lines established from the agar colonies from DNA-treated cells were morphologically normal but replated in agar with higher efficiency than cell lines from control colonies, and 6 of 7 produced tumors on transplantation into hamsters. Only 3 of the 22 cell lines from DNA-treated cell colonies contained SV40 T antigen; lines which did not contain the T antigen transplanted to hamsters as readily as an antigen positive line. Cell lines from control colonies replated in agar with higher efficiency than the parent line, but did not produce tumors in hamsters. The results indicate that SV40 DNA produced an increase in the growth potential of BHK-21/C13 cells which may represent an effect different from that in the usual viral transformation systems.