Use of Trypsin for Isolation of Islets of Langerhans in the Rat

Publisher Website
Google Scholar
Abstract
A new technique for pancreatic islet isolation, based on trypsin administered into the pancreatic duct system and a reduced amount of collagenase for digestion of the removed and chopped pancreatic tissue, yielded viable islets as judged by the metabolic response of 27 inbred, streptozotocin-diabetic rats after intraportal transplantation of the islets: all recipients of > 240 islets normalized their blood glucose, plasma insulin, urine volume and urinary glucose. The number of islets isolated was the same as with the conventional collagenase technique.