KARYOLOGICAL AND ISOENZYME CHARACTERIZATION OF ESTABLISHED HUMAN SARCOMA CELL-LINES

  • 1 January 1980
    • journal article
    • research article
    • Vol. 27  (5) , 557-566
Abstract
Six established human sarcoma cell lines (giant cell tumor of bone B-5GT, fibrosarcoma B-6FS, cystosarcoma phylloides B-19CS, synovial sarcoma U-4SS and osteogenic sarcomas U-20S and U-3930S) were studied and compared to normal B-41FB fibroblastic cells and HeLa cells. For cytogenetic and isoenzyme characterization, conventional and G banding techniques as well as the mobility of lactate dehydrogenase (LDH) and glucose-6-phosphate dehydrogenase (G-6-PD) were employed. All 6 sarcoma cell lines had karyotype and LDH patterns of human cells. A study of the chromosome counts distribution revealed a large variability from one sarcoma cell line to the other. There was no evidence of any cross-contamination between the lines or by HeLa cells. This conclusion is based on the detection of G-6-PD with B phenotype, the lack of HeLa marker chromosomes in all sarcoma lines and the presence of Y chromosome in U-4SS and U-3930S derived from male donors. Each sarcoma cell line revealed a group of distinctive marker chromosomes which can serve to identify them and help to control cell line specificity during in vitro culturing.