Dual-Substrate Plate Diffusion Assay for Proteases
- 1 January 1983
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 45 (1) , 200-204
- https://doi.org/10.1128/aem.45.1.200-204.1983
Abstract
A plate diffusion assay for endopeptidases was developed. Proteases applied to plates containing 1% casein, 1% gelatin, and 1.5% agar caused distinct zones reminiscent of immunoprecipitation bands. The diameter of the zones was linearly proportional to the log of the enzyme activity applied over a range from 0.01 to greater than 100 IU/ml.This publication has 13 references indexed in Scilit:
- The preparation and properties of two new chromogenic substrates of trypsinPublished by Elsevier ,2004
- Manual of Methods for General Bacteriology.Philipp Gerhardt , R. G. E. Murray , Ralph N. Costilow , Eugene W. Nester , Willis A. Wood , Noel R. Krieg , G. Briggs PhillipsThe Quarterly Review of Biology, 1982
- Rapid Screen for Bacteria Degrading Water-Insoluble, Solid Hydrocarbons on Agar PlatesApplied and Environmental Microbiology, 1982
- Effect of Plating Medium on Heat Activation Requirement of Clostridium botulinum SporesApplied and Environmental Microbiology, 1981
- Plate diffusion assay as a rapid method for dosimetry of mutagensApplied and Environmental Microbiology, 1979
- Protease formation by a moderately halophilic Bacillus strain.1974
- A fluorescent assay for proteolytic enzymesAnalytical Biochemistry, 1973
- Isolation and characterization of a protease from Clostridium botulinum type BBiochimica et Biophysica Acta (BBA) - Enzymology, 1972
- The Lowry modification of the Folin reagent for determination of proteinase activityAnalytical Biochemistry, 1965
- Action of rennet and other proteolytic enzymes on casein in casein-agar gelsJournal of Dairy Research, 1963