Staining Pineal Parenchyma by A Modified Hortega Method After Paraffin Embedding
- 1 January 1950
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 25 (1) , 35-38
- https://doi.org/10.3109/10520295009110953
Abstract
Fresh pineal glands are fixed in 10% formalin at room temperature for about 3 days. After washing, dehydrating and clearing they are embedded in paraffin, sectioned and mounted. The tissues are placed in 10% silver nitrate for 24 hours, washed and impregnated in strong silver carbonate. The sections are reduced in 10% formalin, washed and toned in gold chloride, fixed in 5% hyposulfite, counterstained with erythrosin and mounted in Canada balsam. The processes of the pineal parenchymatous cells of the sheep, cow and man have been successfully stained by this method.Keywords
This publication has 1 reference indexed in Scilit:
- The Hortega Silver Carbonate Method for Staining Pituicytes After Paraffin EmbeddingStain Technology, 1943