EFFECT OF ESTRADIOL ON THE ULTRASTRUCTURE OF THE MCF7 HUMAN-BREAST CANCER-CELLS IN CULTURE

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  • 1 January 1982
    • journal article
    • research article
    • Vol. 42  (2) , 667-673
Abstract
The effect was analyzed of estradiol and of 2 classes of antiestrogens on the morphology of the MCF7 human breast cancer cell line by scanning and transmission electron microscopy. Estradiol progressively increased the number and the length of microvilli at the cell surface. The density of the microvilli network increased between 2-11 days of estrogen treatment, while the cells became more globular and less tightly attached to the surface of the dish. Estradiol also progressively transformed cells into secretory cells containing, at day 2, large, clear mitochondria and, at day 4, rough endoplasmic reticulum and Golgi complexes. At day 6, secretory granules (diameter, 0.2 .mu.m), which mainly contained glycoproteins, were first observed in the cytoplasm. By day 8, they were concentrated at the cell membrane and being liberated into the medium. Larger granules (diameter, 0.8 .mu.m), which probably contained lipids, were observed later (day 11). Cell cultures in 10% fetal calf serum not treated by charcoal contained secretory granules. The modifications were induced by physiological concentrations of estradiol but not 5.alpha.-dihydrotestosterone. Progesterone (10 nM for 8 days) completely inhibited the effect of estradiol on the microvilli and secretory activity. Tamoxifen or hydroxytamoxifen [antitumor agents] did not induce secretory activity but did alter the cell morphology compared to control cells. The effects of estradiol were observed in other estrogen receptor-positive breast cancer cell lines (ZR 75-1, T 47 D) but not in an estrogen receptor-negative cell line (BT 20). This morphological evidence that estrogens modify the cell surface of breast cancer cells in culture and transform them into secretory cells complements evidence that they induce the release of a glycoprotein with a MW of .apprxeq. 50,000 into the culture medium (Westley and Rochefort, 1980).