Vasopressin‐stimulated phosphorylation of rat liver phospholipid methyltransferase in isolated hepatocytes
- 17 February 1986
- journal article
- Published by Wiley in FEBS Letters
- Vol. 196 (2) , 274-278
- https://doi.org/10.1016/0014-5793(86)80262-9
Abstract
Addition of vasopressin (1 microM) to isolated rat hepatocytes prelabeled with [32P]phosphate was accompanied by a 250% increase in the phosphorylation of phospholipid methyltransferase. Vasopressin-stimulated phospholipid methyltransferase phosphorylation was time- and dose-dependent. 32P-labeled phospholipid methyltransferase was recovered by immunoprecipitation and SDS-polyacrylamide gel electrophoresis. After electrophoresis, phospholipid methyltransferase was electroeluted from the polyacrylamide gel and subjected to tryptic digestion or HCl hydrolysis. Analysis of 32P-labeled peptides reveals only one site of phosphorylation and the analysis of [32P]phosphoamino acids indicates that phosphoserine is the only labeled amino acid.Keywords
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