Amino Acid Requirements of Growing Mice: Arginine, Lysine, Tryptophan and Phenylalanine

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Abstract
Linear programmed diets designed to maximize the use of proteins and to minimize the use of free (1-) amino acids, and containing five dietary levels of each amino acid under test, were fed to weanling crossbred Carworth Farms No. 1 × Swiss mice in 14-day growth trials. Arginine dietary levels were 0.1, 0.2, 0.3, 0.4 and 0.7%; lysine: 0.2, 0.3, 0.4, 0.5 and 1.0%; tryptophan: 0.03, 0.07, 0.10, 0.13 and 0.17%; and phenylalanine: 0.10, 0.25, 0.40, 0.55 and 0.89%. Growth, feed consumption and regression-adjusted growth rates indicated the following minimum requirements: arginine <0.1% and probably zero, lysine 0.4%, tryptophan 0.1% and phenylalanine 0.4%. The AIN '76 reference diet was included in each amino acid test and resulted in superior growth and feed utilization. It was postulated that the greater content of free amino acids in our diets may have affected the efficiency of feed utilization adversely.