SYNCHRONOUS GROWTH IN LIQUID-MEDIUM OF SPECIES RELATED TO MYCOBACTERIUM RHODOCHROUS NOCARDIA-RESTRICTA AND JENSENIA-CANICRURIA

  • 1 January 1978
    • journal article
    • research article
    • Vol. A129  (3) , 285-293
Abstract
It is possible to obtain a synchronous growth of N. restricta and J. [N] canicruria (species related to M. rhodochrous) in a liquid synthetic medium by inoculating the medium with spherical cells taken during the advanced stationary phase, at the end of the cell division cycle. Good synchrony produces a series of breaks in the slope of culture absorbance and of cell protein content. DNA replication checked either by cell DNA content measurements or by pulse-incorporations of tritiated thymidine, is discontinuous; peaks of incorporation of thymidine follow one another with a periodicity equal to the generation time. A study of the cell division cycle, hitherto unknown, is now possible.