The prosomal protein of 27 kDa and a nuclear 38 kDa protein are immunologically related
Open Access
- 2 July 1990
- journal article
- Published by Wiley in FEBS Letters
- Vol. 267 (1) , 142-146
- https://doi.org/10.1016/0014-5793(90)80309-7
Abstract
A monoclonal antibody, recognizing the prosomal 27 kDa protein, was used to detect prosomal proteins in nuclear fractions. Protein gel electrophoresis and immunoblotting revealed large amounts of a 38 kDa protein and in very low amount a 27 kDa protein reacting with the monoclonal antibody, Immunoblot analysis of V8 protcolytic fragments demonstrated that both proteins arc intimately related and suggest that the nuclear 38 kDa protein could be a precursor of the 27 kDa protein (pros P 27) which is a structural component of cytoplasmic prosomes.Keywords
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