A Rapid Method for the Separation of Guinea Pig IgG1 and IgG2
- 1 January 1974
- journal article
- research article
- Published by Taylor & Francis in Immunological Communications
- Vol. 3 (3) , 285-290
- https://doi.org/10.3109/08820137409057325
Abstract
Immunoelectrophoretically pure IgG1 and IgG2 were isolated from sera of guinea pigs with experimental autoimmune renal tubular disease by chromatography on QAE-Sephadex A-50 (Pharmacia Fine Chemicals, Piscataway, N.J. using a two-step elution. As judged by indirect immunofluorescence tests of the fractions, 70% of the original autoantibody activity to tubular basement membrane was recovered.Keywords
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