DNA sequence analysis of the KM19 locus linked to cystic fibrosis
- 31 July 1990
- journal article
- research article
- Published by Springer Nature in Human Genetics
- Vol. 85 (3) , 319-323
- https://doi.org/10.1007/bf00206754
Abstract
The PstI polymorphism detected by probe KM19 is a highly informative marker in linkage disequilibrium with the cystic fibrosis locus and has been used extensively for prenatal diagnosis. The currently available primers used for polymerase chain reaction- (PCR-) based analysis of this locus have been shown to produce spurious amplification products. In this report, we describe the sequence of the KM19 locus and the major contaminating PCR product. We have used this information to design a more specific amplification procedure for analysis of the KM19 locus.This publication has 16 references indexed in Scilit:
- Identification of the Cystic Fibrosis Gene: Chromosome Walking and JumpingScience, 1989
- Identification of the Cystic Fibrosis Gene: Genetic AnalysisScience, 1989
- Identification of the Cystic Fibrosis Gene: Cloning and Characterization of Complementary DNAScience, 1989
- PRENATAL DIAGNOSIS OF CYSTIC FIBROSIS BY DNA AMPLIFICATION FOR DETECTION OF KM-19 POLYMORPHISMThe Lancet, 1988
- Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA PolymeraseScience, 1988
- Experience with New DNA Markers for the Diagnosis of Cystic FibrosisNew England Journal of Medicine, 1988
- Patterns of polymorphism and linkage disequilibrium for cystic fibrosisGenomics, 1987
- A candidate for the cystic fibrosis locus isolated by selection for methylation-free islandsNature, 1987
- Cystic Fibrosis Locus Defined by a Genetically Linked Polymorphic DNA MarkerScience, 1985
- Localization of cystic fibrosis locus to human chromosome 7cen–q22Nature, 1985