STUDIES ON EFFECTS OF ESCHERICHIA-COLI ENDOTOXIN ON CANALICULAR BILE FORMATION IN ISOLATED PERFUSED RAT-LIVER

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  • 1 January 1977
    • journal article
    • research article
    • Vol. 89  (3) , 471-482
Abstract
The impairment of biliary excretory function induced by Escherichia coli endotoxin in the isolated perfused rat liver was studied by investigating its effects on canalicular bile formation. In experiments where no bile salt was infused, endotoxin administration resulted in significant decreases in bile salt-independent bile flow (BSIF) and sulfobromophthalein (BSP) excretion. It also caused dose-dependent decreases in the transport maximum of BSP but did not alter the ability of the liver to concentrate BSP in the bile. At 2 mg/100 ml it decreased the hepatic removal rate but increased the hepatic storage of the dye. Measurement of 14C-erythritol clearance and bile salt secretion also revealed that endotoxin significantly reduced the BSIF fraction of bile. The primary effects of endotoxin were on BSIF and BSP excretion.