Silencing E1A mRNA by RNA interference inhibits adenovirus replication
- 20 March 2007
- journal article
- research article
- Published by Springer Nature in Archiv für die gesamte Virusforschung
- Vol. 152 (7) , 1305-1314
- https://doi.org/10.1007/s00705-007-0951-z
Abstract
The adenovirus family contains 51 human serotypes, and most human adenoviruses cause widespread respiratory tract infections. Adenovirus infections can result in severe complications in some cases, such as in adenovirus type 11 infection in immunocompromised patients. However, effective treatment methods for adenovirus infections are currently unavailable. This prompted the search for antiviral agents effective against adenovirus infections. In the present study, adenovirus E1A was targeted by RNA interference (RNAi) using synthetic small interfering RNAs (siRNAs) in an attempt to inhibit viral replication, since adenovirus E1A proteins are known to be involved in the transcriptional activation of the viral and cellular genes necessary for controlling the cell cycle and viral replication. The results indicated that the siRNAs effectively reduced the amount of adenovirus E1A mRNA and the levels of replicative intermediates. Additionally, siRNA-mediated gene silencing inhibited adenovirus replication by suppressing the E1A mRNA. These results suggest that the RNAi-mediated targeting of adenovirus E1A may have a potentially therapeutic effect in controlling adenovirus infections.Keywords
This publication has 22 references indexed in Scilit:
- Rhinovirus detection: comparison of real-time and conventional PCRJournal of Virological Methods, 2004
- Silencing SARS‐CoV Spike protein expression in cultured cells by RNA interferenceFEBS Letters, 2004
- Inhibition of human cytomegalovirus replication by small interfering RNAsJournal of General Virology, 2004
- Small interfering RNA molecules as potential anti-human rhinovirus agents: in vitro potency, specificity, and mechanismAntiviral Research, 2004
- Comparative analysis of the genome organization of human adenovirus 11, a member of the human adenovirus species B, and the commonly used human adenovirus 5 vector, a member of species CJournal of General Virology, 2003
- Killing the messenger: short RNAs that silence gene expressionNature Reviews Molecular Cell Biology, 2003
- RNA interference of influenza virus production by directly targeting mRNA for degradation and indirectly inhibiting all viral RNA transcriptionProceedings of the National Academy of Sciences, 2003
- Industrial Accident Compensation Insurance Benefits on Cerebrovascular and Heart Disease in KoreaJournal of Korean Medical Science, 2003
- In Vivo Association of Adenovirus Large E1A Protein with the Human Mediator Complex in Adenovirus-Infected and -Transformed CellsJournal of Virology, 2002
- Application of a gastric cancer cell line (MKN-28) for anti-adenovirus screening using the MTT methodAntiviral Research, 1996