Selective Downregulation of the Angiotensin II AT 1 -Receptor Subtype in Failing Human Ventricular Myocardium
- 4 March 1997
- journal article
- research article
- Published by Wolters Kluwer Health in Circulation
- Vol. 95 (5) , 1193-1200
- https://doi.org/10.1161/01.cir.95.5.1193
Abstract
Background The regulation of angiotensin II receptors and the two major subtypes (AT1 and AT2) in chronically failing human ventricular myocardium has not been previously examined. Methods and Results Angiotensin II receptors were measured by saturation binding of 125I-[Sar1,Ile8]angiotensin II in crude membranes from nonfailing (n=19) and failing human left ventricles with idiopathic dilated cardiomyopathy (IDC; n=31) or ischemic cardiomyopathy (ISC; n=21) and membranes from a limited number of right ventricles in each category. The AT1 and AT2 fractions were determined by use of an AT1-selective antagonist, losartan. β-Adrenergic receptors were also measured by binding of 125I-iodocyanopindolol with the β1 and β2 fractions determined by use of a β1-selective antagonist, CGP20712A. AT1 but not AT2 density was significantly decreased in the combined (IDC+ISC) failing left ventricles (nonfailing: AT1 4.66±0.48, AT2 2.73±0.39; failing: AT1 3.20±0.29, AT2 2.70±0.33 fmol/mg protein; mean±SE). The decrease in ...Keywords
This publication has 21 references indexed in Scilit:
- Characterization of Angiotensin II Receptor Type 2 during Differentiation and Apoptosis of Rat Ovarian Cultured Granulosa CellsBiochemical and Biophysical Research Communications, 1995
- Increased angiotensin-I converting enzyme gene expression in the failing human heart. Quantification by competitive RNA polymerase chain reaction.Journal of Clinical Investigation, 1994
- Differential gene expression and regulation of angiotensin II receptor subtypes in rat cardiac fibroblasts and cardiomyocytes in culture.Journal of Clinical Investigation, 1994
- Angiotensin II (AII)-Induced Myocyte Necrosis: Role of the AII ReceptorJournal of Cardiovascular Pharmacology, 1994
- Cardiac Actions of Angiotensin II: Role of an Intracardiac Renin-Angiotensin SystemAnnual Review of Physiology, 1992
- Role of cardiac angiotensin II in isoproterenol-induced left ventricular hypertrophy.Hypertension, 1992
- Beta-adrenergic neuroeffector abnormalities in the failing human heart are produced by local rather than systemic mechanisms.Journal of Clinical Investigation, 1992
- Nomenclature for angiotensin receptors. A report of the Nomenclature Committee of the Council for High Blood Pressure Research.Hypertension, 1991
- Inhibition of enzymatic degradation of angiotensin II in membrane binding assays: utility of 1,10-phenanthrolineBiochemistry and Cell Biology, 1990
- Decreased Catecholamine Sensitivity and β-Adrenergic-Receptor Density in Failing Human HeartsNew England Journal of Medicine, 1982