Ribosome structure: localization of N6,N6-dimethyladenosine by electron microscopy of a ribosome-antibody complex.
- 1 April 1977
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 74 (4) , 1468-1472
- https://doi.org/10.1073/pnas.74.4.1468
Abstract
Antibodies to the minor nucleoside N6,N6-dimethyladenosine were used to map a unique location of the nucleoside in the small subunit of the Escherichia coli ribosome. Antibodies were induced in rabbits by a nucleoside-bovine albumin conjugate and were highly specific for the dimethyladenosine hapten. The antibodies interacted with 30S ribosomal subunits from strain PR7, but not with subunits from its mutant strain TPR201, which is resistant to kasugamycin and lacks the 2 successive residues of dimethyladenosine normally found near the 3''-end of E. coli 16S rRNA. Electron micrographs of strain PR7 subunits, crosslinked by single Ig[immunoglobulin]G molecules, show a single binding site on the surface of the ribosome. This binding site is consistent with observations relating the 3''-end of the rRNA, binding of initiation factor IF-3 and mRNA, and mapping of specific ribosomal proteins.This publication has 22 references indexed in Scilit:
- Cross-linking of initiation factor IF3 to proteins of the Escherichia coli 30 S ribosomal subunitJournal of Molecular Biology, 1976
- Ribosome structure determined by electron microscopy of Escherichia coli small subunits, large subunits and monomeric ribosomesJournal of Molecular Biology, 1976
- Localization of Escherichia coli Ribosomal Proteins S4 and S14 by Electron Microscopy of Antibody-Labeled SubunitsProceedings of the National Academy of Sciences, 1974
- Location of proteins S5, S13 and S14 on the surface of the 30S ribosomal subunit from Escherichia coli as determined by immune electron microscopyMolecular Genetics and Genomics, 1974
- Ribosome structure: Three‐dimensional distribution of proteins S14 and S4Journal of Supramolecular Structure, 1974
- Immunologic studies on nucleic acids and their components. I. An analysis of the specificity of anti-deoxyadenylate antibodies by a membrane-binding techniqueBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1973
- Identification and isolation of ovalbumin-synthesizing polysomes. I. Specific binding of 125 I-anti-ovalbumin to polysomes.1972
- Mechanism of Kasugamycin Resistance in Escherichia coliNature New Biology, 1972
- DISC ELECTROPHORESIS – II METHOD AND APPLICATION TO HUMAN SERUM PROTEINS*Annals of the New York Academy of Sciences, 1964
- ANTIBODIES SPECIFIC FOR RIBONUCLEOSIDES AND RIBONUCLEOTIDES AND THEIR REACTION WITH DNAProceedings of the National Academy of Sciences, 1964