Azirine ligation: fast and selective protein conjugation via photoinduced azirine–alkene cycloaddition
- 24 September 2010
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Chemical Communications
- Vol. 46 (42) , 7993-7995
- https://doi.org/10.1039/c0cc02863k
Abstract
We report a new bioorthogonal ligation reaction between p-nitrodiphenylazirine and dimethyl fumarate. This photoinduced azirine–alkene cycloaddition provides a rapid (∼2 min) and highly selective route to protein conjugation at neutral pH and room temperature in biological medium.Keywords
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