Hydrogen Peroxide/Oxygen Biosensor Based on Supported Phospholipid Bilayer

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Abstract
The influence of lipid concentration, lipid composition, pH and temperature on the stability and function of metal-supported phospolipid bilayer membranes (s-BLM) was studied and the potential applicability of this system in biosensor development was explored. Parameters to be measured were the resistance as well as the amperometric response of the system to oxygen and hydrogen peroxide. Stability and function of the s-BLM system are inversely related to the lipid concentration. Cholesterol exerts a stabilizing effect at a molar ratio of 4:1 to 1:3 (PC : choiesterol). The effect of stearylamine on membrane stability depends on the sign of the polarizing voltage. Ascorbic acid, but not citric acid nor glutathion nor uric acid interferes with measurements of hydrogen peroxide. The s-BLM is remarkably stable in urine. Its use as a simple but sensitive oxygen sensor for measuring the respiration rate of rat liver mitochondria is demonstrated.