Purification and partial characterization of a cohaemolysin (CAMP-factor) produced byStreptococcus canis
- 1 September 1990
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 64 (2) , 97-101
- https://doi.org/10.1111/j.1574-6968.1990.tb03506.x
Abstract
A cohaemolysin from the culture supernate of a canine pathogenic group G streptococcus (S. canis) was purified to electrophoretic homogeneity. The purification procedure involved ammonium sulphate precipitation, ultrafiltration, gel filtration and preparative isoelectric focusing. The cohaemolysin consisted of a single polypeptide chain, 18.6 kDa, with an isoelectric point at pH 5.1. The protein reacted with an homologous antiserum, appeared to be trypsin-sensitive and relatively heat-stable. The cohaemolysin did not show any non-specific IgG binding activities.Keywords
This publication has 13 references indexed in Scilit:
- Comparison of some Properties of the CAMP-factor from Streptococcus agalactiae with the Haemolytically Latent Active Exosubstance from Streptococcus uberisZentralblatt für Veterinärmedizin Reihe B, 2010
- Unspecific binding of group B streptococcal cocytolysin (CAMP factor) to immunoglobulins and its possible role in pathogenicity.The Journal of Experimental Medicine, 1987
- Identification and characterization of Streptococcus suisVeterinary Microbiology, 1986
- Purification and characterization of camp-factor from streptococcus agalactiae by hydrophobic interaction chromatography and chromatofocusingJournal of Chromatography A, 1985
- Fermenter Growth of Streptococcus agalactiae and Large-scale Production of CAMP FactorMicrobiology, 1983
- A simplification of the protein assay method of Lowry et al. which is more generally applicableAnalytical Biochemistry, 1977
- Purification of penicillinase (β-lactamase) and acid phosphatase from Staphylococcus aureus in one procedureBiochimica et Biophysica Acta (BBA) - Enzymology, 1972
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- The effect on animal erythrocytes of combinations of diffusible substances produced by bacteriaThe Journal of Pathology and Bacteriology, 1964
- A NOTE ON A LYTIC PHENOMENON SHOWN BY GROUP B STREPTOCOCCIImmunology & Cell Biology, 1944