Analysis of the extent of unfolding of denatured insulin‐like growth factor
Open Access
- 1 January 1999
- journal article
- Published by Wiley in Protein Science
- Vol. 8 (7) , 1463-1468
- https://doi.org/10.1110/ps.8.7.1463
Abstract
Insulin‐like growth factor (IGF‐1) contains three disulfide bonds. In the presence of denaturant and thiol catalyst, IGF‐1 shuffles its native disulfide bonds and denatures to form a mixture of scrambled isomers. The composition of scrambled IGF varies under different denaturing conditions. Among the 14 possible scrambled IGF isomers, the yield of the beads‐form isomer is shown to be directly proportional to the strength of the denaturing condition. This paper demonstrates a new approach to quantify the extent of unfolding of the denatured protein.Keywords
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