Excystation In vitro of Echinostoma liei and E. revolutum (Trematoda) Metacercariae
- 1 February 1988
- journal article
- research article
- Published by JSTOR in Journal of Parasitology
- Vol. 74 (1) , 98-102
- https://doi.org/10.2307/3282484
Abstract
Metacercariae of Echinostoma liei and E. revolutum were excysted in an alkaline bile-trypsin medium at 41 C in the absence of acid-pepsin pretreatment. After 60 min at a pH of 7.8 or 8.0, excystation of E. liei reached 98%; optimal excystation of E. revolutum occurred at pH 8.2 and was 70% after 60 min. The rate of excystation was very rapid in E. liei, reaching 91% at 30 min, and less rapid in E. revolutum reaching 40% at 30 min. Almost 100% of the E. liei cysts stored for 5.5 mo at 4 Cin Locke''s 1:1 excysted in the medium, compared to 40% for E. revolutum treated identically.This publication has 1 reference indexed in Scilit:
- Excystation and Cultivation In vitro and In ovo of Cyathocotyle bushiensis (Trematoda) MetacercariaeJournal of Parasitology, 1987