Prokaryotic and eukaryotic monothiol glutaredoxins are able to perform the functions of Grx5 in the biogenesis of Fe/S clusters in yeast mitochondria
- 20 March 2006
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 580 (9) , 2273-2280
- https://doi.org/10.1016/j.febslet.2006.03.037
Abstract
The Saccharomyces cerevisiae monothiol glutaredoxin Grx5 participates in the mitochondrial biogenesis of iron–sulfur clusters. Grx5 homologues exist in organisms from bacteria to humans. Chicken (cGRX5) and human (hGRX5) homologues contain a mitochondrial targeting sequence, suggesting a mitochondrial localization for these two proteins. We have compartmentalized the Escherichia coli and Synechocystis sp. homologues, and also cGRX5 and hGRX5, in the mitochondrial matrix of a yeast grx5 mutant. All four heterologous proteins rescue the defects of the mutant. The chicken cGRX5 gene was significantly expressed throughout the embryo stages in different tissues. These results underline the functional conservation of Grx5 homologues throughout evolutionKeywords
This publication has 32 references indexed in Scilit:
- STRUCTURE, FUNCTION, AND FORMATION OF BIOLOGICAL IRON-SULFUR CLUSTERSAnnual Review of Biochemistry, 2005
- Iron–sulphur cluster biogenesis and mitochondrial iron homeostasisNature Reviews Molecular Cell Biology, 2005
- Iron–sulfur-protein biogenesis in eukaryotesTrends in Biochemical Sciences, 2005
- The Cell's Cookbook For Iron–Sulfur Clusters: Recipes For Fool's Gold?ChemBioChem, 2004
- Glutaredoxins: Glutathione-Dependent Redox Enzymes with Functions Far Beyond a Simple Thioredoxin Backup SystemAntioxidants and Redox Signaling, 2004
- Components involved in assembly and dislocation of iron-sulfur clusters on the scaffold protein Isu1pThe EMBO Journal, 2003
- Grx5 Is a Mitochondrial Glutaredoxin Required for the Activity of Iron/Sulfur EnzymesMolecular Biology of the Cell, 2002
- Thioredoxin and Glutaredoxin IsoformsPublished by Elsevier ,2002
- Iron-sulfur proteins: ancient structures, still full of surprisesJBIC Journal of Biological Inorganic Chemistry, 2000
- Iron-Sulfur Clusters: Nature's Modular, Multipurpose StructuresScience, 1997