Direct automated sequencing of 16S rDNA amplified by polymerase chain reaction from bacterial cultures without DNA purification
Open Access
- 1 November 1992
- journal article
- Published by Oxford University Press (OUP) in Letters in Applied Microbiology
- Vol. 15 (5) , 210-213
- https://doi.org/10.1111/j.1472-765x.1992.tb00765.x
Abstract
The 16S rRNA gene from various bacterial cultures was amplified by the poly-merase chain reaction without DNA purification, and sequenced directly by using a laser fluorescent DNA sequencer and Tth polymerase with a cycle sequencing protocol. The described procedures provide almost complete 16S rDNA sequence data within a couple of days and facilitate systematic studies.Keywords
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