Intramitochondrial localization of the 4-aminobutyrate-2-oxoglutarate transaminase from ox brain

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Abstract
To determine the intramitochondrial location of 4-aminobutyrate transaminase, mitochondria were prepared from ox brain and freed from myelin and synaptosomes by using conventional density gradient centrifugation techniques, and the purity was checked by EM. Inner and outer membranes and matrix were prepared from the mitochondria by large-amplitude swelling and subsequent density-gradient centrifugation. The fractions were characterized by using both EM and different marker enzymes. From the specific activity of the 4-aminobutyrate transaminase in the submitochondrial fractions, this enzyme may be associated with the inner mitochondrial membrane.