Non-H-2 antigens on fibroblasts and an embryocarcinoma cell line react with xenoantisera against H-2 antigens.

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Abstract
The murine embryocarcinoma cell line F9 lacks the classical transplantation antigens. However, rabbit anti-H-2 antigen sera, recognizing backbone structures of H-2 K and D antigens, reacted with 3 types of molecules manufactured by F9 cells. A 49,000- and a 25,000-dalton chain were glycoproteins located on the cell surface. The 3rd component, with an apparent MW of 15,000, and the 25,000-dalton chain seem to be unrelated to the 49,000-dalton glycoprotein. The 25,000- and 15,000-dalton components were not manufactured by splenocytes, thymocytes, hepatocytes or various lymphoma, mastocytoma, and plasmacytoma cell lines. Fibroblasts derived from embryos and adult animals synthesized 25,000- and 15,000-dalton molecules reactive with the rabbit anti-H-2 antigen sera. The 49,000-, 25,000- and 15,000-dalton molecules were not recognized by a syngeneic anti-F9 cell serum.